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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been noticed in many tumors, which leads to chromosome instability, and so might add to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital position in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two further splice variants ended up noted but detailed nucleotide sequence data is just not nonetheless readily available.

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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.

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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible benefits are...

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This information outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide no more bind to your click here epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.

Focus on Information and facts Cyclin E belongs into the highly conserved cyclin family, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is particularly degraded as cells development via S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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